correct gene造句
例句與造句
- shaking flask experiments and hplc analyses showed that 99 isolates still produced four components of avermectins b, in which the yields of avermectins b in 82 isolates were only about 0.1 ~ 2 % of those produced by the parental strain olm73-12.2 of 82 isolates were confirmed to be the correct gene replacement mutants by pcr and sequence analysis . the mutant only producing avermectins bl was not detected
搖瓶發(fā)酵和hplc分析結(jié)果表明,有99個突變株仍然產(chǎn)阿維菌素b的4個組分,其中82株的發(fā)酵單位很低,僅為出發(fā)菌株olm73-12的0.12,從中挑取2株經(jīng)pcr擴增和測序驗證,均發(fā)生了正確的基因取代;沒有檢測到僅產(chǎn)b1的突變株,這表明阿維菌素b2組分的產(chǎn)生并不是因為阿維菌素pks上dh2的部分活性所造成。 - western blot analysis showed that rhpk-5 ( recombinant human plasminogen kringle 5, rhpk-5 ) protein was recognized by mab same as native hpk-5 . the result suggested that we obtained correct gene sequence of hpk-5 and got the purified rhpk-5 . section ii : construction of pbv220 / hpk-5 vector for obtaining high-level hpk-5 expression system, the hpk-5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk-5
coli)作為宿主,經(jīng)sds-page分析,篩選表達量最高的菌株作為發(fā)酵用工程菌株;用western-blot方法鑒定hpk-5因子的免疫學活性;用搖瓶發(fā)酵的方法,研究發(fā)酵培養(yǎng)基的體積(溶解氧)、組成成份及誘導起始時間和誘導持續(xù)時間對目的蛋白表達量的影響,優(yōu)化hpk-5基因工程菌的表達條件。 - It's difficult to find correct gene in a sentence. 用correct gene造句挺難的